Stem Cells Handbook
Format: PDF / Kindle (mobi) / ePub
This book discusses critical areas of progress in stem cell research, including the most recent research and applications of pluripotent embryonic cells, induced pluripotent cells, oligopotent tissue stem cells and cancer stem cells. The text covers basic knowledge of stem cell biology, stem cell ethics, development of techniques for applying stem cell therapy, the technology of obtaining appropriate cells for transplantation as well as the role of stem cells in cancer and how therapy may be directed to cancer stem cells. This new volume is essential reading for all scientists currently in the field or allied research areas, and those for those graduate students who envision a career in stem cells.
requires a 1.1- to 1.3-fold higher miR-302 concentration than that found in human ESCs (approximately 0.9–1.0 million copies per ESC) . As shown in Fig. 1, such a high cellular miR-302 concentration induces both global DNA demethylation and co-expression of Oct4, Sox2, and Nanog in human iPSCs [9, 10]. The expression of Lin28 and many other ESC marker genes was also observed 1–3 days following Oct4-Sox2-Nanog co-expression. In human ESCs, induced miR-302 expression over the normal level was
pathophysiological conditions seemingly trigger proliferation in previously quiescent or postmitotic astrocytes. Indeed, genetic fate mapping combined with viral vector injections demonstrated that the proliferating reactive astrocytes are not derived from the NSC niche of the SVZ or SGZ, but rather originate in the local grey matter of the cerebral cortex [24, 211]. Interestingly, the proliferative reaction of astrocytes occurs with a delay (5–7 days after the insult) compared to microglia
of cocarcinogenic action and related phenomena. Cancer Res. 1941;1:807. 69. Boutwell RK. Some biological aspects of skin carcinogenesis. Prog Exp Tumor Res. 1964;4:207–50.PubMed 70. Van Duuren BL, Sivak A, Katz C, Seidman I, Melchionne S. The effect of ageing and interval between primary and secondary treatment in two-stage carcinogenesis on mouse skin. Cancer Res. 1975;35:502–5.PubMed 71. Potten CS, Morris RJ. Epithelial stem cells in vivo. J Cell Sci Suppl. 1988;10:45–62.PubMedCrossRef
potential and capacity of hepatic progenitors to restore functional liver mass. In addition to the role of stem cells in liver regeneration, we will also review the contribution of these cells to the development of hepatocellular carcinoma. Finally we will discuss the future direction of the field of hepatic stem/progenitor cells and the potential therapeutic use of these cells in liver disease. Introduction The concept of bipotent hepatic progenitor cells residing in the cholangioles, the
Smith A. Embryonic stem cells. In: Marshak DR, Gardner RL, Gottlieb D, editors. Stem cell biology. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press; 2001. p. 205–30. 44. Nichols J, Chambers I, Taga T, Smith A. Physiological rationale for responsiveness of mouse embryonic stem cells to gp130 cytokines. Development. 2001;128:2333–9.PubMed 45. Morrison-Graham K, Takahashi Y. Steel factor and c-kit receptor: from mutants to a growth factor system. Bioessays. 1993;15(2):77–83.PubMed